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![]() | Immunoblotting Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-CRMP2 (Cat. No. C2993). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:10,000 Lanes 1,6: Mouse brain (S1 fraction) 2: Rat brain (S1 fraction) 3: HeLa cells 4: HEK-293T cells transfected with human CRMP2 5: Untransfected HEK-293T cells 6: Inhibition with CRMP2 immunizing peptide (human 476-493) (10 μg/mL) |
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C2993
Sigma
Anti-CRMP2 antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Collapsin Response Mediator Protein 2, Anti-Dihydropyriminidase-like 2 (DPYSL2), Anti-TOAD-64, Anti-Dihydropyriminidase Related Protein-2 (DRP2, DRP-2, DHPRP2) |
Description
| Immunogen | synthetic peptide corresponding to amino acids 476-493 located near the C-terminus of human CRMP2, conjugated to KLH. This sequence is identical in mouse and rat CRMP2. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~62 kDa |
| species reactivity | rat, mouse, human |
| concentration | ~1.5 mg/mL |
| application(s) | immunoblotting: 0.1-0.2 μg/mL using HeLa whole cell lysate and mouse brain extract (S1 fraction) |
| Gene Information | human ... DPYSL2(1808) |
| rat ... Dpysl2(25416) | |
| mouse ... Dpysl2(12934) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |







