C2993

Immunoblotting
Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-CRMP2 (Cat. No. C2993). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:10,000
Lanes
1,6: Mouse brain (S1 fraction)
2: Rat brain (S1 fraction)
3: HeLa cells
4: HEK-293T cells transfected with human CRMP2
5: Untransfected HEK-293T cells
6: Inhibition with CRMP2 immunizing peptide (human 476-493) (10 μg/mL)

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C2993

Sigma

 

Anti-CRMP2 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym:Anti-Collapsin Response Mediator Protein 2, Anti-Dihydropyriminidase-like 2 (DPYSL2), Anti-TOAD-64, Anti-Dihydropyriminidase Related Protein-2 (DRP2, DRP-2, DHPRP2)

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Description

Immunogensynthetic peptide corresponding to amino acids 476-493 located near the C-terminus of human CRMP2, conjugated to KLH. This sequence is identical in mouse and rat CRMP2.
Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody formaffinity isolated antibody
clone(polyclonal)
formbuffered aqueous solution
mol wtantigen mol wt ~62 kDa
species reactivityrat, mouse, human
concentration~1.5 mg/mL
application(s)immunoblotting: 0.1-0.2 μg/mL using HeLa whole cell lysate and mouse brain extract (S1 fraction)
Gene Informationhuman ... DPYSL2(1808)
 rat ... Dpysl2(25416)
 mouse ... Dpysl2(12934)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3